Mutant strains of Desulfovibrio vulgaris str. We investigated the effects of the first two enzymatic steps in the dissimilatory sulfate reduction (DSR) network – sulfate permease and sulfate adenylyl transferase (Sat) – on the sulfur and oxygen isotopic composition of residual sulfate. The use of stable isotopes to trace biogeochemical sulfur cycling relies on an understanding of how isotopic fractionation is imposed by metabolic networks. 3Division of Biology and Biomedical Sciences, Washington University in St.2Department of Earth and Planetary Sciences, Harvard University, Cambridge, MA, United States.
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